BarcodingĮach oligo comprises two barcoding components and one RNA-binding component. These gel beads are coated with oligonucleotides, which are short nucleotide sequences. The system divides individual cells into tiny water droplets and adds reagents and barcoded gel beads. Hence, before sequencing can take place, you must dissociate each sample into single-cell suspensions by a tissue-specific protocol.Īfter loading, this is what happens inside the 10x Chromium System: Partition into droplets With samples, what is meant is a single-cell suspension. Each chip can hold multiple samples simultaneously. The 10x Chromium system works with microfluidic chips. These function as tiny reaction chambers in which the RNA molecules from each cell receive barcoded labels and prepare for sequencing. By using tiny vessel-like tubes, the system can partition individual cells or nuclei into micro-scale water droplets. How it worksġ0x Chromium works with microfluidics. It is upgradable to ‘full-range’ 10x Chromium X with a software update. The 10x Genomics Chromium iX is a “light” version that is more cost-effective and has lower throughput in the same machine. In comparison: the previous 10x Genomics instrument, the Chromium Controller, can run low-throughput (100–1000 cells) and moderate-throughput (1,000–10,000 cells) assays. It offers the highest throughput (> 100,000 cells and more) per run and is compatible with the newest single-cell assays. The most advanced generation of 10x Chromium instruments is the Chromium X Series. Read on to learn about the various Chromium series, how the platform works, and what its features are. 10x Chromium is compatible with the droplet-based scRNA-seq methods of 10x Genomics. Also, it can operate at a scale of hundreds to ten thousands of cells per run. ![]() ![]() It enables the analysis of gene expression profiles at single-cell resolution. Home > 10x Chromium: how does it work and what are its features?ġ0x Chromium is a machine, or platform, that researchers use for high-throughput single-cell RNA sequencing (scRNA-seq). Support Sample requirements, shipping, and more.Getting started All the steps for starting your project.Blog Thought-leadership, knowledge, and updates.Resource Library Webinars, our selection tool, and more.Case studies Detailed insights into past projects.Publications From our clients and project work.Data analysis Options beyond the included exploratory analysis. ![]() Our approach An inside look at SCD’s White-Glove approach.Research Areas Find out how single-cell sequencing is advancing disease understanding and drug development in your research area of interest.Applications Overview From fundamental biology to translational research and drug development, single-cell sequencing applications are revolutionizing science.Research & Development Our expert team drives innovation and versatility.Bulk RNA sequencing Detect transcriptome profiles in populations.VASA-seq Full-length total-RNA sequencing.10x Genomics High-throughput single-cell solutions.SORT-seq Plate-based single-cell transcriptomics.Services Overview Explore multiple single-cell, spatial and bulk RNA sequencing platforms to best suit your biological question.
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